FIG 8.

A synthetic CD4 mimetic modulates binding of nnAbs and bNAbs and ADCC. (A) CEM-NKR cells infected with T/F HIV-1 (strain CH058) were incubated with mCD4.2-PS1 (10 nM) for 10 min before measurement of Ab binding by flow cytometry. Data from one representative experiment (out of 3) are shown. (B) CEM-NKR cells infected with T/F HIV-1 (strain CH058) and primary CD4 T cells infected with HIV strain KB18v from patient KB18 were incubated or not with mCD4.2-PS1 before measurement of Ab binding by flow cytometry. nnAbs (top) (in blue) and bNAbs are depicted separately. Radar plots represent the mean percentages (plain lines) and standard deviations (dashed lines) of Ab⁺ cells among infected (Gag⁺) cells evaluated by flow cytometry, from 3 independent experiments. (C and D) ADCC was evaluated in the presence or absence of mCD4.2-PS1 by using CEM-NKR cells infected with T/F HIV-1 (strain CH058) (C) or CD4 T cells infected with HIV-1 strain KB18v (D). Results are means ± standard errors of the means with NK cells from three donors (*, P < 0.05; **, P < 0.005 [by a Mann-Whitney test]).