TABLE 1.
Primers used in this study
| Expt and primer name | Primer sequence (5′–3′) |
|---|---|
| Plasmid construction | |
| IE1-Cas9_F | GACAAGAGACGTCGCTAGCGGCCACCATGGACAAGAAGTACTCCAT |
| IE1-Cas9_R | CTGATTATGATCTAGAGTCGTCACACCTTCCTCTTCTTCTTGGGGT |
| U6-2_F | AGGTTATGTAGTACACATTG |
| U6-2_R | ACTTGTAGAGCACGATATTTTG |
| ie1-sg1-R | GCTATTTCTAGCTCTAAAACGTTGCCGATGGTTGGTTCACACTTGTAGAGCACGATATTTTG |
| ie1-sg2-R | GCTATTTCTAGCTCTAAAACAATACTTTAATATTTGACTCACTTGTAGAGCACGATATTTTG |
| me53-sg1-R | GCTATTTCTAGCTCTAAAACAATTATTTCAAACACTTTGCACTTGTAGAGCACGATATTTTG |
| me53-sg2-R | GCTATTTCTAGCTCTAAAACCCGCGTCGGGTCTGGTGCACACTTGTAGAGCACGATATTTTG |
| U6-sgRNA-R | AAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
| Dimer1_Bsa1-F | ATGCGGTCTCCTGACCCTAGGAGGTTATGTAGTACACATTG |
| Dimer1_Bsa1-R | ATGCGGTCTCGTAGGAAAAAAGCACCGACTCGGTGCCACT |
| Dimer2_Bsa1-F | ATGCGGTCTCACCTAAGGTTATGTAGTACACATTG |
| Dimer2_Bsa1-R | ATGCGGTCTCGCAAGAAAAAAGCACCGACTCGGTGCCACT |
| Dimer3_Bsa1-F | ATGCGGTCTCACTTGAGGTTATGTAGTACACATTG |
| Dimer3_Bsa1-R | ATGCGGTCTCGTAATAAAAAAGCACCGACTCGGTGCCACT |
| Dimer4_Bsa1-F | ATGCGGTCTCAATTAAGGTTATGTAGTACACATTG |
| Dimer4_Bsa1-R | ATGCGGTCTCGGAGTCCTAGGAAAAAAGCACCGACTCGGTGCCACT |
| Relative BmNPV gene copy no. detection | |
| gp64-qPCR-F | CTTTAATGAGCAGACACGCAG |
| gp64-qPCR-R | CTTTAATGAGCAGACACGCAG |
| lef3-qPCR-F | CGGAAGAGGTAGAACGGTCA |
| lef3-qPCR-R | GTGCGAGGCTAAAGAAAACG |
| BmRP49-qPCR-F | AAACATACAAGATGGCTATAAGACCTG |
| BmRP49-qPCR-R | TTTATAAATGACATGTGAACATACCTC |
| Relative BmNPV gene expression level detection | |
| ie1-qRT-F | GCTCAAGACCACTGATAATCTC |
| ie1-qRT-R | AATCGTCCAAGTATTCGTCCA |
| me53-qRT-F | TCTTTAACTTCCGTCGTGTG |
| me53-qRT-R | ACGATTCTGACCGCATAGAC |
| ie0-qRT-F | ATGATAAGAACCAGCAGTCAC |
| ie0-qRT-R | CTTTGCGTCGATTTGTATGTC |
| p143-qRT-F | TGGCTTCATACTTTAGCAACC |
| p143-qRT-R | GTTTGACGATGACAACCACAG |
| vp39-qRT-F | TCTAAATCTCAATTCCTCCGTG |
| vp39-qRT-R | GCATTCTAGACACCACAAACC |
| p10-qRT-F | CCATTGCGGAAACTAACACA |
| p10-qRT-R | AGCAGTGTCACCGGTCAATA |
| Bmrp49-qRT-F | TCAATCGGATCGCTATGACA |
| Bmrp49-qRT-R | ATGACGGGTCTTCTTGTTGG |
| Mutagenesis detection | |
| ie1-F | GTGTTCGCCATTAGGGCAGTA |
| ie1-R | GCGCACCAACTCCCATTGTT |
| me53-F | GGTCTACCTCTAACAGCGTCG |
| me53-R | TAAAGCCTCTCGATGGCTGA |
| Inverse PCR | |
| Inverse PCR-F1 | CAGTGACACTTACCGCATTGA |
| Inverse PCR-R1 | CATTTTGACTCACGCGGTC |
| Inverse PCR-F2 | CGCTATTTAGAAAGAGAGAGCAA |
| Inverse PCR-R2 | ATCACGTAAGTAGAACATGAAATAACA |
| Off-target assay | |
| ie1-s1-OT1-F | CTTGGACCAATTAGGACGTGG |
| ie1-s1-OT1-R | GAGTCTCGTGCGCCATTTGT |
| ie1-s1-OT2-F | ACTGTACATCATGCACGGGG |
| ie1-s1-OT2-R | CCCACCCCAAATCAATACGC |
| ie1-s1-OT3-F | ATTCAGTGCGTCACGTAGGG |
| ie1-s1-OT3-R | GAATATGCAGTGCGAAGGCG |
| ie1-s2-OT1-F | CAGACCGCAAAGCTCACAGA |
| ie1-s2-OT1-R | TTTGCTACCTAACGCCTCCA |
| ie1-s2-OT2-F | ACGCCTCTAAGTCCAAGTCC |
| ie1-s2-OT2-R | GACTTCGGTAGGGCAAGGAT |
| ie1-s2-OT3-F | GGGTCGTATAGCCGAACTGG |
| ie1-s2-OT3-R | GCGACTCGAAAACAGCTTCC |
| me53-s1-OT1-F | AAGGAACTGTCGGCGAAAAGA |
| me53-s1-OT1-R | GCGAGCGTCTTGTGTGAGAG |
| me53-s1-OT2-F | GACTGTCGCTAGTGAGGAGC |
| me53-s1-OT2-R | TCGCCCGCACATACATACAA |
| me53-s1-OT3-F | TGATGCGTTAAAAGCGCGAG |
| me53-s1-OT3-R | GTGGATAGTCGGCCAGATCG |
| me53-s2-OT1-F | CGAGAAGTCAAGCTGAGCGA |
| me53-s2-OT1-R | ATGTCCGGAGTCCAGTAGCA |
| me53-s2-OT2-F | TGCCCGCAGCTTGTAAGTAT |
| me53-s2-OT2-R | GTTGGAAGGGGCAGACTTGA |
| me53-s2-OT3-F | TTCTTTCTCCGTGCACACCA |
| me53-s2-OT3-R | GTGTTCGTTTGCCTGTCGAG |