Plasmid construction |
|
IE1-Cas9_F |
GACAAGAGACGTCGCTAGCGGCCACCATGGACAAGAAGTACTCCAT |
IE1-Cas9_R |
CTGATTATGATCTAGAGTCGTCACACCTTCCTCTTCTTCTTGGGGT |
U6-2_F |
AGGTTATGTAGTACACATTG |
U6-2_R |
ACTTGTAGAGCACGATATTTTG |
ie1-sg1-R |
GCTATTTCTAGCTCTAAAACGTTGCCGATGGTTGGTTCACACTTGTAGAGCACGATATTTTG |
ie1-sg2-R |
GCTATTTCTAGCTCTAAAACAATACTTTAATATTTGACTCACTTGTAGAGCACGATATTTTG |
me53-sg1-R |
GCTATTTCTAGCTCTAAAACAATTATTTCAAACACTTTGCACTTGTAGAGCACGATATTTTG |
me53-sg2-R |
GCTATTTCTAGCTCTAAAACCCGCGTCGGGTCTGGTGCACACTTGTAGAGCACGATATTTTG |
U6-sgRNA-R |
AAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
Dimer1_Bsa1-F |
ATGCGGTCTCCTGACCCTAGGAGGTTATGTAGTACACATTG |
Dimer1_Bsa1-R |
ATGCGGTCTCGTAGGAAAAAAGCACCGACTCGGTGCCACT |
Dimer2_Bsa1-F |
ATGCGGTCTCACCTAAGGTTATGTAGTACACATTG |
Dimer2_Bsa1-R |
ATGCGGTCTCGCAAGAAAAAAGCACCGACTCGGTGCCACT |
Dimer3_Bsa1-F |
ATGCGGTCTCACTTGAGGTTATGTAGTACACATTG |
Dimer3_Bsa1-R |
ATGCGGTCTCGTAATAAAAAAGCACCGACTCGGTGCCACT |
Dimer4_Bsa1-F |
ATGCGGTCTCAATTAAGGTTATGTAGTACACATTG |
Dimer4_Bsa1-R |
ATGCGGTCTCGGAGTCCTAGGAAAAAAGCACCGACTCGGTGCCACT |
Relative BmNPV gene copy no. detection |
|
gp64-qPCR-F |
CTTTAATGAGCAGACACGCAG |
gp64-qPCR-R |
CTTTAATGAGCAGACACGCAG |
lef3-qPCR-F |
CGGAAGAGGTAGAACGGTCA |
lef3-qPCR-R |
GTGCGAGGCTAAAGAAAACG |
BmRP49-qPCR-F |
AAACATACAAGATGGCTATAAGACCTG |
BmRP49-qPCR-R |
TTTATAAATGACATGTGAACATACCTC |
Relative BmNPV gene expression level detection |
|
ie1-qRT-F |
GCTCAAGACCACTGATAATCTC |
ie1-qRT-R |
AATCGTCCAAGTATTCGTCCA |
me53-qRT-F |
TCTTTAACTTCCGTCGTGTG |
me53-qRT-R |
ACGATTCTGACCGCATAGAC |
ie0-qRT-F |
ATGATAAGAACCAGCAGTCAC |
ie0-qRT-R |
CTTTGCGTCGATTTGTATGTC |
p143-qRT-F |
TGGCTTCATACTTTAGCAACC |
p143-qRT-R |
GTTTGACGATGACAACCACAG |
vp39-qRT-F |
TCTAAATCTCAATTCCTCCGTG |
vp39-qRT-R |
GCATTCTAGACACCACAAACC |
p10-qRT-F |
CCATTGCGGAAACTAACACA |
p10-qRT-R |
AGCAGTGTCACCGGTCAATA |
Bmrp49-qRT-F |
TCAATCGGATCGCTATGACA |
Bmrp49-qRT-R |
ATGACGGGTCTTCTTGTTGG |
Mutagenesis detection |
|
ie1-F |
GTGTTCGCCATTAGGGCAGTA |
ie1-R |
GCGCACCAACTCCCATTGTT |
me53-F |
GGTCTACCTCTAACAGCGTCG |
me53-R |
TAAAGCCTCTCGATGGCTGA |
Inverse PCR |
|
Inverse PCR-F1 |
CAGTGACACTTACCGCATTGA |
Inverse PCR-R1 |
CATTTTGACTCACGCGGTC |
Inverse PCR-F2 |
CGCTATTTAGAAAGAGAGAGCAA |
Inverse PCR-R2 |
ATCACGTAAGTAGAACATGAAATAACA |
Off-target assay |
|
ie1-s1-OT1-F |
CTTGGACCAATTAGGACGTGG |
ie1-s1-OT1-R |
GAGTCTCGTGCGCCATTTGT |
ie1-s1-OT2-F |
ACTGTACATCATGCACGGGG |
ie1-s1-OT2-R |
CCCACCCCAAATCAATACGC |
ie1-s1-OT3-F |
ATTCAGTGCGTCACGTAGGG |
ie1-s1-OT3-R |
GAATATGCAGTGCGAAGGCG |
ie1-s2-OT1-F |
CAGACCGCAAAGCTCACAGA |
ie1-s2-OT1-R |
TTTGCTACCTAACGCCTCCA |
ie1-s2-OT2-F |
ACGCCTCTAAGTCCAAGTCC |
ie1-s2-OT2-R |
GACTTCGGTAGGGCAAGGAT |
ie1-s2-OT3-F |
GGGTCGTATAGCCGAACTGG |
ie1-s2-OT3-R |
GCGACTCGAAAACAGCTTCC |
me53-s1-OT1-F |
AAGGAACTGTCGGCGAAAAGA |
me53-s1-OT1-R |
GCGAGCGTCTTGTGTGAGAG |
me53-s1-OT2-F |
GACTGTCGCTAGTGAGGAGC |
me53-s1-OT2-R |
TCGCCCGCACATACATACAA |
me53-s1-OT3-F |
TGATGCGTTAAAAGCGCGAG |
me53-s1-OT3-R |
GTGGATAGTCGGCCAGATCG |
me53-s2-OT1-F |
CGAGAAGTCAAGCTGAGCGA |
me53-s2-OT1-R |
ATGTCCGGAGTCCAGTAGCA |
me53-s2-OT2-F |
TGCCCGCAGCTTGTAAGTAT |
me53-s2-OT2-R |
GTTGGAAGGGGCAGACTTGA |
me53-s2-OT3-F |
TTCTTTCTCCGTGCACACCA |
me53-s2-OT3-R |
GTGTTCGTTTGCCTGTCGAG |