FIG 3.
rRABV-mBAFF improves the speed and magnitude of anti-RABV G antibody responses. C57BL/6 mice were immunized i.p. (A, B, and C) or i.m. (D, E, and F) with a single dose of 105 FFU/mouse of rRABV or rRABV-mBAFF. On the indicated days postimmunization, RABV G-specific IgM (A and D) and IgG (B and E) antibodies were determined by ELISA. (C and F) VNA titers were determined by RFFIT on pooled serum of immunized mice. Neutralization titers, defined as the inverse of the highest serum dilution that neutralizes 50% of the challenge virus (challenge virus strain 11), were normalized to international units/milliliter (IU/ml) using the WHO antirabies virus antibody reference standard. (A, B, and C) n = 9, i.e., 3/group from 3 independent experiments; (D, E, and F) n = 3/group from a single experiment. An unpaired, two-tailed t test was used to compare groups (*, P < 0.05; **, P < 0.01; ****, P < 0.0001). OD 490 nm indicates the optical density at 490 nm.