FIG 5.

Ribosome recruitment to the CP IRES. (A) The “land and scan” model of initiation suggests that 40S ribosomal subunits land upstream of the start codon before scanning in the 5′-to-3′ direction. Three AUG codons were individually introduced into the −1 frame upstream of the CP in full-length TCV genomic RNA. Translation initiating at a −1 frame AUG terminates within the CP coding region (labeled “−1 STOP”). The percentage of ribosome entry within each region is from the data in panel B. The ORFs for expression from AUG-1 to -3 are shown. (B) In vitro translation of AUG-1 to -3 mutants. CP expression data are shown as mean percentages of the WT level and standard deviations. Data from three independent experiments were normalized to p28 levels.