FIG 8.

Effect of metalloprotease inhibition on MHV infection is S strain specific. L2 cells were treated with batimastat (50 μM) and/or bafilomycin A (100 nM) or DMSO as described in Materials and Methods (1.5% DMSO final concentration for all conditions) and infected with isogenic chimeric viruses bearing the S protein from JHM.SD, A59, or MHV-2 (MOI, 0.5). Two-way ANOVA showed significant effects of treatment, strain, and interaction (P < 0.0001 for all); the asterisks represent the results of Tukey's multiple-comparison tests between each pair of viruses under each condition (level of significance for at least 2 of the 3 comparisons; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). Data are representative of 2 independent experiments with n = 5 technical replicates.