TABLE 1.
Tmprss2 mRNA expression in mouse cell linesa
| Sample | CT (Actb) | CT (Tmprss2) | No. of reactions with CT (Tmprss2) ≤ 40b | ΔCT |
|---|---|---|---|---|
| Reference RNA | 17.9 | 24.3 | NA | 6.3 |
| Kidney | 22.3 | 27.4 | NA | 5.1 |
| Prostate | 20.1 | 25.9 | NA | 5.8 |
| DBT | 16.5 (0.3) | 39.1 (1.1) | 4/9 | 22.6 (1.0) |
| 17Cl1 | 16.0 (0.2) | 38.3 (1.6) | 6/9 | 22.3 (1.5) |
| L2 | 16.3 (0.1) | 33.5 (0.3) | 9/9 | 17.2 (0.3) |
a
Threshold cycle (CT) values for cell lines are the means (standard deviations) from n = 3 cDNA samples prepared from separate wells of each cell type; each cDNA sample was then assayed in triplicate (9 reactions total).
b
The final cycle number (40) was used for the Tmprss2 CT value where no signal was otherwise detected. Data are representative of two independent experiments. NA, not applicable.