Table 3.
Thorough comparison of SPR immunosensor and the ELISA immunosensor.
| SPR | ELISA | ||
|---|---|---|---|
| LOD | 0.09 ng/mL | 0.21 | |
| Linear Range | 0.3–32 ng/mL | 0.3–32 ng/mL | |
| Solid matrix | Matrix | CM5 chip | Microwells |
| Reusability | Yes (>50 times) | No | |
| Reagents consumption | RCT-BSA | 110 μL in total | 100 μL/sample |
| Antibody I | 80 μL/sample | 50 μL/sample | |
| Antibody II/HRP-antibody II | 80 μL/sample | 100 μL/sample | |
| TMB | - | 100 μL/sample | |
| Analysis time | Immobilization time | 11 min | Overnight |
| Blocking time | 7 min | 2 h | |
| Competition time | 4 min | 45 min | |
| Signal amplification time | 4 min | 45 min | |
| Automation | Yes | No | |
| Labeling | Antibody I | No | No |
| Antibody II | No | HRP | |