Fig. 3.

The effects of haloperidol (5 and 20 µM), olanzapine (5 and 20 µM) and amisulpride (20 and 100 µM) on VPAC2 expression in T98 cell line. Cells were incubated with neuroleptic drugs for 24 h (a, b) or 72 h (c, d) and subjected to gene expression studies. Control cells were treated with medium. 1 µg of total RNA isolated from cells was reverse transcribed and 50 ng of cDNA was used for the real-time PCR analysis. All real-time PCR reactions were performed in duplicate, using ACTB gene as an endogenous normalization factor. Results are expressed as relative units (r.u.)—a number resulting from the normalization procedure; values are the mean ± SEM (n = 8). Statistical differences are shown as *p < 0.05, **p < 0.01 and ***p < 0.001