Fig. 8.

SP600125 decreased the VPA-mediated increase in the neuronal differentiation of mouse embryonic NSCs and the neurite outgrowth of NSC-derived neurons. a Summary of the proportions of mouse embryonic NSCs-derived β-tubulin-III-, MAP-2-, CNPase-positive cells, and GFAP-positive cells. b Summary of the quantification of β-tubulin-III, MAP-2, CNPase, and GFAP gene expression. c Summary of the quantification of BDNF, GDNF and CDNF gene expression. d Quantification of the number of primary neurites and branches and the number of neurites. e Quantification of the number of branch points. f Quantification of longest neurite length and total neurite length. *p < 0.05 compared with the VPA-treated control cultures; ^#p > 0.05 compared with the VPA-treated control cultures; ^##p > 0.05 compared with the VPA-treated control cultures