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. 2017 Feb 27;42(4):1267–1278. doi: 10.1007/s11064-016-2168-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Effects of EP4 receptor on the activation of microglia after SAH. Iba-1 staining for microglias showed activation of EP4 by AE1-329 attenuated the number and activation of microglias in the brain 24 h after SAH while inhibition of EP4 by AE3-208 showed opposite effects (a, b). Microglia was indicated by Iba-1 staining and nuclear was indicated by DAPI staining. Bar: 25 µm. Data were expressed as mean ± SEM (n = 8, ^###p < 0.001 vs. Sham, *p < 0.05 vs. SAH treated with vehicle,***p < 0.001 vs. SAH treated with vehicle)