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The Akt inhibitor LY294002 reverses the protective effects exerted by aFGF on OGD-treated TJ and AJ proteins in endothelial cells, as demonstrated using immunofluorescence staining. A-D. Immunofluorescence staining of a confluent endothelial cell monolayer for claudin-5, occludin, β-catenin and p120-catenin. Nuclei were labeled by DAPI. The proteins with clear bright signals are labeled. Magnification is 40×.
