Figure 4.

HoxB9 regulates TGF-β1 expression in OSCC cells. A. Cal27 cells were treated with siRNA targeting HoxB9, and the TGF-β1, Smad2, p-Smad2, Slug and Snail levels were determined. GAPDH was used as an internal standard for protein loading. B. SCC-25 cells were treated with siRNA targeting HoxB9, and the TGF-β1, Smad2, p-Smad2, Slug and Snail levels were determined. GAPDH was used as an internal standard for protein loading. The values are presented as the means ± SEM. One-way ANOVA with post-Dunnett analysis was performed using GraphPad Prism 5.0. *P < 0.05, **P < 0.01 versus the control group (n = 3).