Figure 1.

The effect of cisplatin on the mRNA expression of COX-1 and COX-2 in mesangial cells. A, B. qRT-PCR was used to measure the mRNA expression of COX-1 in mesangial cells after cisplatin treatment. The dose- and time-dependent experiments were performed, respectively. C, D. qRT-PCR was used to determine the mRNA level of COX-2 in mesangial cells following cisplatin treatment. The dose- and time-dependent experiments were performed, respectively. In time-dependent studies, the MCs were treated with cisplatin at a dose of 40 μM. N=3-4 for each group. *P<0.05 vs. control; **P<0.01 vs. control; ***P<0.001 vs. control.