Figure 2.

Clusterin protects against STS-induced apoptosis in tubule cultures. (A) Induction of caspase3/7 activity by STS was concentration-dependent with a maximum at 0.5 µM. (B) Pre-treatment with recombinant CLU followed by treatment with 0.5 µM STS for 24 hrs completely blocked caspase3/7 activity in contrast to STS alone. (C) Increasing concentrations of STS showed an increased phosphatidylserine externalization. (D) CLU nearly completely blocked STS-induced phosphatidylserine externalization. (A-D) *P < 0.05 significantly different from control; #P < 0.05 significantly different from STS alone. In (A-D) results are expressed as percentage of control (Ctrl = DMSO-treated tubules) and the means ± SEMs of 4 independent experiments are shown (A-D).