Figure 3.

Localization of ApoER2/VLDLR and signalling via Akt in rat testis. (A) ApoER2 can be found modestly in Sertoli cells (arrow, SC) and pachytene spermatocytes (arrow, pSpc), but strongly in Leydig cells (B). (C) VLDLR is localized in pachytene spermatocytes (arrow, pSpc) and strongly in Leydig cells (D). Magnification ×40. An example for a negative control is shown in Figure 4F. (E) Tubules were stimulated with CLU (2 µg/ml), RAP (200 nM), RAP+CLU or vehicle alone (Ctrl, control) for 24 hrs and Akt phosphorylation (P-Akt) was quantified. RAP completely inhibited CLU-induced Akt phosphorylation in the tubules. Results are expressed as x-fold of control. The means ± SEMs of 3 independent experiments in duplicates are shown. *P < 0.05 significantly different from control; #P < 0.05 significantly different from CLU alone.