Figure 4.

Localization of meiosis-specific proteins in adult rat testis. A. Stra8 was detected strongly in spermatogonia (Spg, arrows), round (rSpd, arrow) and elongated spermatids (eSpd, arrow). In pachytene spermatocytes (pSpc) Stra8 staining was weak. B. In mouse testis, Stra8 was found strongly in spermatogonia (Spg, arrow). C. MYBL1 was localized in primary spermatocytes (Spc I, arrow). D. CREB was detected faintly in Sertoli cells (SC, arrow) and pachytene spermatocytes, but strongly in round spermatids (rSpd, arrow), and peritubular cells (PC, arrow). E. LDHC protein was found in Leydig cells, only very weak in pachytene spermatocytes, but abundantly in round spermatids (rSpd, arrow) and spermatozoa. F. One negative control without secondary antibody is exemplarily shown. Magnification A, ×40; C-F, ×25.