Table 1.
Premars of related genes in realtime PCR
| Sequence (5’→3’) | Length | Tm | Location | ||
|---|---|---|---|---|---|
| APP | Forward Primer | TCCGAGAGGTGTGCTCTGAA | 20 | 62.4 | 860-879 |
| Reverse Primer | CCACATCCGCCGTAAAAGAATG | 22 | 61.8 | 974-953 | |
| PSEN1 | Forward Primer | TGCACCTTTGTCCTACTTCCA | 21 | 61 | 15-35 |
| Reverse Primer | GCTCAGGGTTGTCAAGTCTCTG | 22 | 62.5 | 145-124 | |
| REST | Forward Primer | GGCAGATGGCCGAATTGATG | 20 | 61.5 | 212-231 |
| Reverse Primer | CTTTGAGGTCAGCCGACTCT | 20 | 61.2 | 298-279 | |
| HHIP | Forward Primer | GAAGATGCTCTCGTTTAAGCTGC | 23 | 61.8 | 6-28 |
| Reverse Primer | CCACCACACAGGATCTCTCC | 20 | 61.3 | 212-193 | |
| CNTN2 | Forward Primer | TTGGACCCGTCTTTGAAGAGC | 21 | 62.3 | 110-130 |
| Reverse Primer | TACTGGGTTAGAGGCTAGGCA | 21 | 61.5 | 357-337 | |
| APOC2 | Forward Primer | ATGGGGTCTCGGTTCTTCCT | 20 | 62.2 | 2-21 |
| Reverse Primer | GTCTTCTGGTACAGGTCTTTGG | 22 | 60 | 176-155 | |
| GAPDH | Forward Primer | AGGTCGGTGTGAACGGATTTG | 21 | 62.6 | 8-28 |
| Reverse Primer | GGGGTCGTTGATGGCAACA | 19 | 62.6 | 102-84 | |
| GM13498 | Forward Primer | GACCCGTCAGGGACCAAAAC | 20 | 62.7 | 163-182 |
| Reverse Primer | AACGGTAAGGAATCACGATGTG | 22 | 60.4 | 349-328 | |
| 1700030L20RIK | Forward Primer | GGTGGCTGTTTTATGTCCCAA | 21 | 60.5 | 774-794 |
| Reverse Primer | CAACCACACCATTGTTGAGGA | 21 | 60.4 | 885-865 | |
| AK038159 | Forward Primer | CAGGTCTTCTTCAAACAACTGCT | 23 | 60.9 | 470-492 |
| Reverse Primer | TGCTTTCTCGGGAAGTCTGGA | 21 | 62.9 | 560-540 | |
| DQ113493 | Forward Primer | TGTCTGTGCGAGATGCAAC | 19 | 60.4 | 1514-1532 |
| Reverse Primer | CCATAGTGGGGTCATGCGAG | 20 | 62.1 | 1621-1602 | |
| U6 | Forward Primer | ACCCTGAGAAATACCCTCACAT | 22 | 60.2 | 140-161 |
| Reverse Primer | GACGACTGAGCCCCTGATG | 19 | 61.8 | 201-183 |