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. 2017 Mar 31;12(3):e0174271. doi: 10.1371/journal.pone.0174271

Fig 1. Changes in glycolysis, EMT markers, and ROS production after matrix detachment.

Fig 1

(A) AR Huh-BAT cells had higher expression of HK II, p-PDH, rGCS, and MCT-1 compared to Huh-BAT and HepG2 cells at the indicated time points after matrix detachment. (B) AR HepG2 cells had higher expression of HK II, p-PDH, rGCS, and MCT-1 compared to Huh-BAT and HepG2 cells at the indicated time points after matrix detachment. (C) Expression of Snail was potentiated in AR Huh-BAT and AR HepG2 cells compared to Huh-BAT and HepG2 cells, respectively. (D) Lactic acid production in AR Huh-BAT and AR HepG2 cells was significantly increased compared to that in Huh-BAT and HepG2 cells, respectively. (E) Glutathione production in AR Huh-BAT and AR HepG2 cells was significantly increased compared to that in Huh-BAT and HepG2 cells, respectively. (F) Production of reactive oxygen species in AR Huh-BAT and AR HepG2 cells was significantly decreased compared to that in Huh-BAT and HepG2 cells, respectively. *P<0.05; ***P<0.001. Abbreviation: Att, attached; AR, anoikis-resistant; GSH, glutathione; HK II, hexokinase II; h, hours; MCT-1, monocarboxylate transporter-1; p-PDH, phosphorylated pyruvate dehydrogenase; rGCS, gamma-glutamylcysteine synthetase; ROS, reactive oxygen species.