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. 2017 Mar 31;12(3):e0174271. doi: 10.1371/journal.pone.0174271

Fig 6. Apoptosis of Huh-BAT, HepG2, and the corresponding AR cells after 3-BP, BSO, and a combination treatment.

Fig 6

(A) Apoptosis rates of Huh-BAT, HepG2, and the corresponding AR cells receiving the indicated treatments were evaluated by annexin V-FITC staining. The upper panel depicts the proportion of apoptotic cells, and the lower panel shows the quantitative results. (B) PARP expression in Huh-BAT, HepG2, and the corresponding AR cells receiving the indicated treatments was analyzed. At a combination treatment, 3-BP (40 μM) was treated for 48 hours at 24 hours after BSO treatment (200 μM) in the cells. *P<0.05; **P<0.01; ***P<0.001. Abbreviation: AR, anoikis-resistant; Att, attached; BSO, buthionine sulfoximine; Co., control; PARP, poly ADP-ribose polymerase; 3-BP, 3-bromopyruvate.