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. 2017 Mar 31;12(3):e0174271. doi: 10.1371/journal.pone.0174271

Fig 7. Suppression of HCC invasion by 3-BP, BSO, and a combination treatment.

Fig 7

(A) Invasion capability of AR Huh-BAT cells significantly suppressed by BSO (200 μM), 3-BP (20 μM) and a combination by invasion assay using Boyden chambers (quantified at right panels). (B) Invasion capability of AR HepG2 cells significantly suppressed by BSO (200 μM), 3-BP (20 μM) and a combination by invasion assay using Boyden chambers (quantified at right panels). The concentrations of 3-BP (20 μM) and BSO (200 μM) which did not kill cancer cells were used in this assay. 3-BP and BSO was treated for 72 hours. In a combination treatment in this assay, 3-BP (20 μM) was treated for 72 hours with 24-hour pre-treatment of BSO (200 μM). *P<0.05; **P<0.01; ***P<0.001. Abbreviation: AR, anoikis-resistant; BSO, buthionine sulfoximine; 3-BP, 3-bromopyruvate.