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. 2017 Mar 28;112(6):1236–1245. doi: 10.1016/j.bpj.2017.02.012

Figure 1.

Figure 1

Mechanotransduction-induced phenotypic transition in PC3 cells. (a) qRT-PCR analysis of E-cad, N-cad, AGR2, ALDH3A1, and GDF15 expression for PC3 cells cultured under different conditions as indicated for 72 h. Rigid adherent substrates included TCP (Dish), flat PDMS (PMA-flat), and PDMS micropost array (PMA) with a post height of 0.7 μm (PMA-0.7). Soft culture conditions included PMA with a post height of 14.5 μm (PMA-14.5) and suspension (Susp) culture. (b) Immunoblot assays of N-cad, AGR2, ALDH3A1, GDF15, and lamin A/C in PC3 cells grown under different conditions as indicated for 72 h. E-cad was undetectable by immunoblot. (c). Paclitaxel IC-50 value determined by nonlinear regression analysis of cell viability curve (measured by WST-1) against concentrations of paclitaxel. PC3 cells cultured on TCP dish and in suspension had IC-50 values of 5 and 24 nM, respectively. (d). Trypan blue exclusion assays to determine viability of PC3 cells grown on TCP dish (27 ± 4%) or in suspension (44 ± 3%) treated with 25 nM paclitaxel for 48 h. Vehicle controls (−) were included for comparison. Data represents the mean ± SE with n = 3. P-values were calculated using Student’s t-test. ∗p < 0.05.