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. 2017 Mar 27;11:955–968. doi: 10.2147/DDDT.S128795

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The in vitro cell growth inhibition effect resulted from MTT assay on (A) L-02, (B) SMMC-7721 and (C) HepG2 cells, after being treated separately for 24 h with various concentrations of OA and OA-MVLs.

Notes: The solvent of DMSO (0.25%) was used as vehicle controls. A490 was measured after the MTT incubation. Each data point represents mean ± SD based on five repetitive determinations in three independent experiments. The significance of the differences was evaluated using Student’s t-test (*P<0.05, **P<0.01), and the asterisk marks represent the difference of OA vs control and OA-MVLs vs control, respectively.

Abbreviations: MTT, 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; OA, oleanolic acid; MVLs, multivesicular liposomes; DMSO, dimethyl sulfoxide; SD, standard deviation.