Table 3.

Comparison of the observed and expected values obtained by constraints applied on Y₁ and Y₂

Variables	Optimized values	Response	Mean values expected	Mean values observed	Bias* (%)
Cholesterol:soybean lecithin (X1)	0.57	Y1	95.96	93.19	2.89
Total lipids:drug (X2)	10
Triolein:soybean lecithin (X3)	0.38	Y2	12.02	11.57	3.75

Notes: Y₁, encapsulation efficiency (%); Y₂, mean particle size (µm).

^*Bias (%) was calculated as: (predicted value – observed value)/predicted value ×100 (n=3).