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. 2014 Jul 29;4:5870. doi: 10.1038/srep05870

Figure 3. The selectivity of RHP-2 for sulphide.

Figure 3

(A) Fluorescence responses of RHP-2 (5 μM) towards Na2S (100 μM) and various biothiols after 40 min of incubation. 1.Na2S (0 μM); 2.Na2S (10 μM); 3.Na2S (100 μM); 4.Cys (100 μM); 5.Cys (1 mM); 6.Hcy (100 μM); 7.Hcy (1 mM); 8.GSH (1 mM); 9.GSH (10 mM); 10.Na2S (10 μM) + Cys (100 μM); 11.Na2S (100 μM) + Cys (100 μM); 12.Na2S (10 μM) + Cys (1 mM); 13.Na2S (100 μM) + Cys (1 mM); 14.Na2S (10 μM) + Hcy (100 μM); 15.Na2S (100 μM) + Hcy (100 μM); 16.Na2S (10 μM) + Hcy (1 mM); 17.Na2S (100 μM) + Hcy (1 mM); 18.Na2S (10 μM) + GSH (1 mM); 19.Na2S (100 μM) + GSH (1 mM); 20.Na2S (10 μM) + GSH (10 mM); and 21.Na2S (100 μM) + GSH (10 mM). (B) Fluorescence spectra of RHP-2 (5 μM) with Na2S (100 μM) and various amino acids (1 mM) after 40 min of incubation. (C, D) Fluorescence responses of RHP-2 (5 μM) towards Na2S (100 μM), ROS (1 mM), RNS (1 mM), sulphur-containing inorganic ions (1 mM), reducing agents (1 mM), inorganic salts (1 mM) and S-nitroso-glutathione (SNG, 1 mM) after 40 min of incubation. Data are presented as the mean ± SD (n = 3).