Table 1.
(A) CTD Peptides with Localized Phosphosites from Positive Mode UVPD Analysisa and (B) CTD Peptides with Ambiguous Phosphosites from Positive Mode UVPD Analysis
| A | ||
|---|---|---|
| sequence | heptad # (phosphosite in heptad) | construct |
| TASSPGGASPNYSPSSPNYSPTpSPLY | 6 (S5) | full |
| YApSPRYASTTPNFNPQSTGY | 7 (S5) | full |
| ASPRYASTpTPNFNPQSTGY | 8 (T5) | full |
| SPTpSPVYpSPTVQF | 11 (S5), 12 (S2) | full |
| QSpSPSFAGSGSNIY | 13 (S5) | full |
| QSSPSFAGSGSNIYpSPGNAY | 15 (S2) | full |
| SPGNAYpSPSSSNY | 16 (S2) | full |
| SASPQYSPApSPAYSQTGVKY | 26 (S5) | full |
| SPApSPAYSQTGVKY | 26 (S5) | full |
| SPApSPKYSPTSPL | 32 (S5) | CTD4 |
| YSPSSPQHSPSNQYpSPTGSTY | 36 (S2) | full |
| SATpSPRYSPNMSIYSPSSTKY | 37 (S5) | full |
| SATSPRYpSPNMSIY | 38 (S2) | full, CTD4 |
| pSPSSTKY | 39 (S2) | full |
| SPTpSPTYpTPTARNY | 40 (S5), 41 (T2) | full |
| SPTSPTYpTPTARNY | 41 (T2) | full, CTD4 |
| SPTpSPMYSPTAPSHY | 42 (S5) | full, CTD4 |
| SPTpSPMYpSPTAPSHY | 42 (S5), 43 (S2) | full |
| SPTpSPAYSPSpSPTFEESED | 44 (S5), 45 (S5) | full |
| B | ||
|---|---|---|
| sequence | heptad # (phosphosite in heptad) | construct |
| TASSPGGASPNYp(SPSS) PNYSPTpSPLY | 5 (S2/S4/S5), 6 (S5) | Full |
| p(SPTS)PTYSPPSPSY | 28 (S2/T4/S5) | CTD4 |
| p(SPTS)PAYSPSSPTFEESED | 44 (S2/T4/S5) | Full, CTD4 |
a
A sequence coverage map is provided in Supporting Information Table 2.