Table 2.
(A) CTD Peptides with Localized Phosphosites from Negative Mode UVPD Analysisa and (B) CTD Peptides with Ambiguous Phosphosites from Negative Mode UVPD Analysis
| A | ||
|---|---|---|
| sequence | heptad # (phosphosite in heptad) | construct |
| SPSpSPNYSPTpSPLY | 5 (S5), 6 (S5) | Full, CTD2, CTD3 |
| SPSSPNYSPTpSPLY | 6 (S5) | Full, CTD2, CTD3 |
| SPTpSPVYpSPTVQF | 11 (S5), 12 (S2) | Full, CTD2, CTD3 |
| SPTSPVYpSPTVQF | 12(S2) | Full, CTD2, CTD3 |
| QSpSPSFAGSGSNIY | 13 (S5) | Full, CTD2, CTD3 |
| QSSPSFAGSGSNIYpSPGNAY | 15 (S2) | Full |
| SPApSPAYSQTGVKY | 26 (S5) | Full, CTD4 |
| SPApSPKYSPTSPL | 32 (S5) | CTD4 |
| SIYpSPSSTKY | 39 (S2) | CTD4 |
| SPTSPMYpSPTAPSHY | 43 (S2) | Full, CTD4 |
| SPTpSPAYSPSpSPTFEESED | 44 (S5), 45 (S5) | CTD4 |
| SPTSPAYSPSpSPTFEESED | 45 (S5) | Full, CTD4 |
| B | ||
|---|---|---|
| sequence | heptad # (phosphosite in heptad) | construct |
| ASp(TT)PNFNPQSTGY | 8 (T4/T5) | Full, CTD2, CTD3 |
| SPp(TS)PVYSPTVQF | 11 (T4/S5) | Full, CTD2, CTD3 |
| SAp(TS)PRYpSPNMSIY | 37 (T4/S5), 38 (S2) | CTD4 |
| SPp(TS) PAYSPSSPTFEESED | 44 (T4/S5) | Full, CTD4 |
a
A sequence coverage map provided in Supporting Information Table 2.