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Un-stretched kinetochores showed no separation between components. (A) The Cartesian coordinates of Mtw1 and Dam1 of kinetochore 2 (KT2 in Fig. 9A). (B, C) The positions of Dam1 relative to Mtw1 in Cartesian (B) and polar (C) coordinates. (D) The positions of Dam1 relative to Mtw1 form a cluster. Gray points show raw positions and red points show the same data after smoothing with a 1-second window average. Dashed line marks a circle of 44 nm radius, as in Figure 9F.
