Table 1. Primers for Sanger sequencing.
Primer | Location* | Sequence (5'-3') | Samples† |
---|---|---|---|
P1bE1E2sens | 697–718 | TGGGTAAGGTCATCGATACCCT | All except c14, c16, c17 |
HCV1bextsens | 821–841 | CGGCGTGAACTATGCAACAGG | All except c3, c14, c15, c16, c17, c37 |
HCV1bintsens | 843–873 | CACCATGGGTTGCTCTTTCTCTATCTTCC | c6, c12, c20, c27, c37, c42 |
VDA8505sens | 1178–1196 | CCTGTGCGGGTCGTTTTT | All except c14, c16, c17 |
seq12sens | 1290–1309 | CGCATGGCTTGGGATATGAT | c34, c36 |
HVR1extsens | 1290–1310 | CGCATGGCGTGGGACATGATG | c15 |
VDA8505antisens | 1435–1454 | AACCTTAGCCCAGTTCCCCG | All except c14, c16, c17 |
IICantisens | 1605–1620 | ACCCAGTGCTATTCAT | All except c14, c15, c16, c17, c37, c42 |
GY16N1550sens | 2247–2266 | GTGGAGCACAGGTTCGAAGC | All except c14, c16, c17, c37, c41 |
HCV1bintantisens | 2559–2584 | AATCAGGCCTCAGCCTAGGCTATCTG | c12, c20, c27, c37, c42 |
P1bE1E2antisens | 2775–2794 | CACGATGCAGCCATCTCCCG | All except c14, c16, c17 |
* Location indicate nucleotide postion relative to the H77 genome
† Samples sequencing with each primer