Table 4. Comparaison of ambiguous position with two sequencing techniques according to the percentage of MiSeq nucleotide variation.
| MiSeq sequencing | Sanger sequencing | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample | Location* | Depth | A | C | G | T | % variation† | Double peak | Primers‡ |
| c15 | 1008 | 1114 | 1032 | 0 | 82 | 0 | 7,4 | "-" | 1 |
| c12 | 2082 | 1736 | 0 | 0 | 80 | 1656 | 4,6 | "+" | 2 |
| c2 | 826 | 1615 | 0 | 68 | 0 | 1547 | 4,2 | "-" | 3 |
| c15 | 1322 | 1681 | 1617 | 0 | 64 | 0 | 3,8 | "-" | 1 |
| c12 | 1858 | 1723 | 0 | 62 | 0 | 1661 | 3,6 | "-" | 1 |
| c12 | 2291 | 2104 | 70 | 0 | 2034 | 0 | 3,3 | "+" | 1 |
| c5 | 1274 | 1856 | 0 | 1798 | 0 | 58 | 3,1 | "+" | 4 |
| c3 | 2191 | 3735 | 3636 | 0 | 99 | 0 | 2,7 | "-" | 2 |
| c5 | 1811 | 3209 | 3129 | 0 | 80 | 0 | 2,5 | "-" | 2 |
| c10 | 1603 | 2531 | 2472 | 0 | 59 | 0 | 2,3 | "-" | 2 |
| c2 | 2558 | 3174 | 72 | 0 | 3102 | 0 | 2,3 | "-" | 3 |
* Location indicate nucleotide postion relative to the H77 genome.
† % nucleotide variation at this postion after MiSeq analysis.
‡ Number of nucleotide read at this position according to the numbers of primers used.
"+" Double peak clearly visible on electrophoregram at this position.
"+/-" Double peak no clearly visible on electrophoregram at this position.
"-" No double peak on electrophoregram at this position.