Fig. 4.

Simulated effect of chloroquine on net synthesized DNA, obtained by integrating the rate of DNA synthesis (Fig. 3) over the intraerythrocytic development cycle. The ordinate shows values of net synthesized DNA (•) relative to control values as a function of chloroquine dose. The net amount of synthesized DNA decreases as the dose increases (red symbols). The amount of synthesized DNA returns back to control levels (green symbols) when $\alpha$ is set to 1 for the core genes in Table S3, while simulating the effect of chloroquine on metabolic fluxes. Control values of net synthesized DNA (•) were obtained from transcriptome data of Pf3D7 or PfDd2 (Llinas et al., 2006) (Table S2). In the legend, CTL denotes DNA synthesized under control conditions after chloroquine treatment, whereas -CG denotes that after chloroquine treatment with $\alpha$ set to 1 for core genes. The results were averaged across twenty independent simulations. Error bars represent the standard error associated with each prediction. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)