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. 2017 Mar 31;12(3):e0174896. doi: 10.1371/journal.pone.0174896

Table 1. Requirements and technical solution applied in the new intermittent hypoxia system.

Requirements Technical solutions
Maximum adjustability in the design of the experimental protocols in terms of hypoxia levels and exposure times Controller enabling setting two different oxygen levels and two time periods
Fast transition between different oxygen levels to allow short hypoxia periods Dual sensor input to the controller board
Mixing antechamber with oxygen sensor
Small volume
High gas flow
Precise oxygen control in the chamber Mixing antechamber with oxygen sensor
Dual-sensor input to the controller board
Use of Techniplast GM500 cage with patented sealing design
Evenly distributed gas flow throughout the chamber Modification of Techniplast GM500 cage with patented ergonomic design
Minimizing animal suffering System compatible with the housing cage
Humidifier
Muffler system
Standard housing space for animals