Fig. 1.

Adult skin fibroblasts isolated from Mst1^−/− mice exhibit a higher proliferation rate.

A) Western blot analysis showing the complete absence of Mst1 in Mst1^−/− skin fibroblasts. B) Representative images of EdU incorporation assay. Nuclei were stained for EdU incorporation (green) and DAPI (blue). Scale bars = 100 μm. C) Quantification of EdU positive cells showed that adult skin fibroblasts lacking Mst1 displayed a higher proliferation rate than wild type cells (**P < 0.01, n = 3 biological replicates, i.e. fibroblasts were isolated from 3 independent mice in each group, one way ANOVA followed by post-hoc multiple comparisons).