Fig. 3.

Mst1^−/− iPSC exhibit higher proliferation rates and survival.

A) Images of EdU incorporation assay on Mst1^−/− and WT iPSC in normal media containing 20% FBS and in starving media (0% FBS). B) Quantification of EdU positive cells indicated that iPS cells lacking Mst1displayed higher proliferation rate in both normal and starving media (*P < 0.05, **P < 0.01, n = 3 independent iPSC in each group, one way ANOVA followed by post-hoc multiple comparisons). C) Measurement of cell viability using Trypan blue staining demonstrated that following treatment with CoCl₂ for 16 h Mst1^−/− iPSC showed higher viability compared to WT iPSC (**P < 0.01, n = 3 independent iPSC populations, one way ANOVA followed by post-hoc multiple comparisons). D) Analysis of Caspase 3/7 activity indicated that Mst1^−/− cells displayed lower caspase activity in both basal condition and after treatment with CoCl₂ for 16 h (*P < 0.05, **P < 0.01, n = 3 independent iPSC populations, one way ANOVA followed by post-hoc multiple comparisons).