Figure 6.

NR2C Antagonism Decouples Pre-synaptic and Cytosolic [Ca²⁺]_i in SCN Neurons

(A and B) (A) Circadian profiles (mean ± SEM, n = 5) of SCN slices co-transduced with SyF::GCaMP3 and Syn-RCaMP1h AAVs to detect pre-synaptic or cytosolic [Ca²⁺]_i, respectively. Gray area shows higher nighttime fluorescence of pre-synaptic [Ca²⁺]_i in comparison to cytosolic [Ca²⁺]_i and circadian variation of pre-synaptic/cytosolic neuronal [Ca²⁺]_i ratios (B).

(C) Representative traces of SyF::GCaMP3 and Syn-RCaMP1h fluorescence in SCN slices before and during DQP-1105 treatment.

(D) Mean ± SEM of baseline SyF::GCaMP3 and Syn-RCaMP1h fluorescence in the presence of DQP-1105.

(E) Mean ± SEM ratios of cytosolic/pre-synaptic baseline fluorescence before DQP-1105 and in the presence of the drug (paired two-tailed t test; ^∗p < 0.05, n = 5).

(F) Cartoon depicting the proposed model for the astrocytic-neuronal intercellular axis in the dorsal SCN.