Figure 7.

SCN Astrocytes Control Spatiotemporal Wave of Circadian Gene Expression in Juvenile Slices

(A) Schematic of strategy, with floxed Ck1ε^Tau/Tau PER2::LUC SCN transduced with AAVs encoding Cre recombinase driven by Syn or GFAP promoters to assess the effects of neuronal and astrocytic Ck1ε^Tau/Tau knockout on circadian rhythms.

(B) Representative PMT traces of PER2::LUC SCN from neuronal- or astrocyte-restricted Ck1ε^Tau/Tau knockout (mean ± SEM, p < 0.0001, n = 4).

(C) Bar graphs showing period of PER2::LUC, neuronal, and astrocytic [Ca²⁺]_i circadian oscillations co-detected in neuronal- or astrocyte-restricted Ck1ε^Tau/Tau knockout, respectively.

(D) Poincaré plots revealing CoL dorsalization in astrocytic-restricted Ck1ε^Tau/Tau knockout, within a single SCN (left) and across different SCNs (right, n = 3; ANOVA). SCN outlined for presentation purposes.

Bar graphs are mean ± SEM; n experimental replicates as depicted on bars. Statistical test is as follows: two-way repeated-measures ANOVA, Bonferroni corrected; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001. dpt, days post-transduction. See also Movie S3.