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. 2017 Mar 22;93(6):1420–1435.e5. doi: 10.1016/j.neuron.2017.02.030
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-GFAP antibody, Chicken polyclonal to GFAP Abcam ab4674; RRID: AB_304558
Anti-Aldh1L1 antibody, Rabbit polyclonal to Aldh1L1 Abcam ab87117; RRID: AB_10712968
Anti-GAD65 + GAD67, Rabbit polyclonal to GAD65 + GAD67 Abcam ab49832; RRID: AB_880149
Anti-EAAT3 (EAAC1), Goat polyclonal to EAAC1 Millipore AB1520; RRID: AB_90732
Anti-Ng2, Rabbit polyclonal to Ng2 Abcam ab83178; RRID: AB_10672215
Anti-Iba1, Rabbit polyclonal to Iba1 Wako Chemicals 019-19741; RRID: AB_839504
Anti-AVP, Rabbit polyclonal to AVP Bachem T4562.0400; RRID: AB_518671
Alexa Fluor 647 Goat Anti-Chicken antibody Thermo Fisher Scientific A-21449; RRID: AB_2535866
Alexa Fluor 488 Goat Anti-Rabbit antibody Thermo Fisher Scientific A-11008; RRID:AB_143165
Alexa Fluor 488 Goat Anti-Chicken antibody Thermo Fisher Scientific A-11039; RRID: AB_142924
Alexa Fluor 647 Donkey Anti-Goat antibody Thermo Fisher Scientific A-21447; RRID: AB_141844
Alexa Fluor 488 Chicken Anti-Rabbit antibody Thermo Fisher Scientific A-21441; RRID: AB_141735
Alexa Fluor 647 Goat Anti-Rabbit antibody Thermo Fisher Scientific A-21245; RRID: AB_141775

Chemicals, Peptides, and Recombinant Proteins

DL-TBOA (DL-threo-β-Benzyloxyaspartic acid) Tocris Bioscience Cat. No. 1223; CAS No 205309-81-5
UCPH-101 (2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile) Tocris Bioscience Cat. No. 3490; CAS No 1118460-77-7
WAY 213613 N-[4-(2-Bromo-4,5-difluorophenoxy)phenyl]-L-asparagine Tocris Bioscience Cat. No. 2652; CAS No: 868359-05-1
DQP-1105 5-(4-Bromophenyl)-3-(1,2-dihydro-6-methyl-2-oxo-4-phenyl-3-quinolinyl)-4,5-dihydro-γ-oxo-1H-pyrazole-1-butanoic acid Tocris Bioscience Cat. No. 4491; CAS No: 380560-89-4
QNZ-46 4-[6-Methoxy-2-[(1E)-2-(3-nitrophenyl)ethenyl]-4-oxo-3(4H)quinazolinyl]benzoic acid Tocris Bioscience Cat. No. 4801; CAS No: 1237744-13-6
TCN-201 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl)hydrazino]carbonyl]benzyl]benzenesulfonamide Tocris Bioscience Cat. No. 4154; CAS No: 852918-02-6
(+)-MK 801 maleate (5S,10R)-(+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate Tocris Bioscience Cat. No. 0924; CAS No: 77086-22-7
MSO L-S-(3-Amino-3-carboxypropyl)-S-methylsulfoximine Sigma-Aldrich Cat. No. M5379; CAS No: 15985-39-4

Experimental Models: Organisms/Strains

Grin2ctm1CreERT2_EGFP/Wtsi Wellcome Trust Sanger Institute MGI:5632501
Ck1e Tau (B6.129-Csnk1etm1Asil/J) Jax Laboratories RRID: IMSR_JAX:016183
PER2::LUC (B6.129S6-Per2tm1Jt/J) Jax Laboratories RRID: IMSR_JAX:006852

Recombinant DNA

Syn.RCaMP1h.WPRE.SV40 Penn Vector Core AV-1-PV3010
hSyn.GCaMP3.WPRE.SV40 Penn Vector Core AV-1-PV1627
hSyn.iGluSnFr.WPRE.SV40 Penn Vector Core AV-1-PV2723
GFAP.iGluSnFr.WPRE.SV40 Penn Vector Core AV-1-PV2726
hSyn.ArcLightD.WPRE.SV40 Penn Vector Core AV-1-36857P
GfaABC1D.PI.cyto-GCaMP3.SV40 Penn Vector Core AV-5-44331
GfaABC1D.Lck-GCaMP6f.SV40 Penn Vector Core AV-5-PV3107
AAV1.CMV.PI.SynGCaMP3.SV40 Penn Vector Core AV-1-PV2368
CAG.Flex.tdTomato.WPRE.bGH Penn Vector Core AV-1-ALL864
hSyn.eGFP.WPRE.bGH Penn Vector Core AV-1-PV1696
GFAP.eGFP.WPRE.bGH Penn Vector Core AV-5-PV2407
EF1a.flex.EYFP.WPRE.hGH Penn Vector Core AV-1-27056
GFAP-hM3DGq:mCherry UNC Vector Core N/A
GFAP-mCherry:Cre UNC Vector Core N/A
Syn-mCherry:Cre UNC Vector Core N/A
GFAP-EGFP:Cre UNC Vector Core N/A
AAV-EF1a-flex-taCasp3-TEVp UNC Vector Core N/A

Software and Algorithms

BioDare (Moore et al., 2014) https://www.biodare.ed.ac.uk
Clock Lab Analysis 6 ActiMetrics http://actimetrics.com/products/clocklab/
Prism 6 GraphPad http://www.graphpad.com/scientific-software/prism/
Igor Pro WaveMetrics https://www.wavemetrics.com/products/igorpro/igorpro.htm
SARFIA Semi-Automated Routines for Functional Image Analysis (Igor Plugin) (Dorostkar et al., 2010) http://www.igorexchange.com/node/1723
Axograph X Axograph Scientific http://www.axograph.com/
Neuromatic (Igor plugin) Jason Rothman, UCL http://neuromatic.thinkrandom.com
Oriana 4 Kovacs Computer Services https://www.kovcomp.co.uk/oriana/