Antibodies |
|
Anti-GFAP antibody, Chicken polyclonal to GFAP |
Abcam |
ab4674; RRID: AB_304558
|
Anti-Aldh1L1 antibody, Rabbit polyclonal to Aldh1L1 |
Abcam |
ab87117; RRID: AB_10712968
|
Anti-GAD65 + GAD67, Rabbit polyclonal to GAD65 + GAD67 |
Abcam |
ab49832; RRID: AB_880149
|
Anti-EAAT3 (EAAC1), Goat polyclonal to EAAC1 |
Millipore |
AB1520; RRID: AB_90732
|
Anti-Ng2, Rabbit polyclonal to Ng2 |
Abcam |
ab83178; RRID: AB_10672215
|
Anti-Iba1, Rabbit polyclonal to Iba1 |
Wako Chemicals |
019-19741; RRID: AB_839504
|
Anti-AVP, Rabbit polyclonal to AVP |
Bachem |
T4562.0400; RRID: AB_518671
|
Alexa Fluor 647 Goat Anti-Chicken antibody |
Thermo Fisher Scientific |
A-21449; RRID: AB_2535866
|
Alexa Fluor 488 Goat Anti-Rabbit antibody |
Thermo Fisher Scientific |
A-11008; RRID:AB_143165
|
Alexa Fluor 488 Goat Anti-Chicken antibody |
Thermo Fisher Scientific |
A-11039; RRID: AB_142924
|
Alexa Fluor 647 Donkey Anti-Goat antibody |
Thermo Fisher Scientific |
A-21447; RRID: AB_141844
|
Alexa Fluor 488 Chicken Anti-Rabbit antibody |
Thermo Fisher Scientific |
A-21441; RRID: AB_141735
|
Alexa Fluor 647 Goat Anti-Rabbit antibody |
Thermo Fisher Scientific |
A-21245; RRID: AB_141775
|
|
Chemicals, Peptides, and Recombinant Proteins |
|
DL-TBOA (DL-threo-β-Benzyloxyaspartic acid) |
Tocris Bioscience |
Cat. No. 1223; CAS No 205309-81-5 |
UCPH-101 (2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile) |
Tocris Bioscience |
Cat. No. 3490; CAS No 1118460-77-7 |
WAY 213613 N-[4-(2-Bromo-4,5-difluorophenoxy)phenyl]-L-asparagine |
Tocris Bioscience |
Cat. No. 2652; CAS No: 868359-05-1 |
DQP-1105 5-(4-Bromophenyl)-3-(1,2-dihydro-6-methyl-2-oxo-4-phenyl-3-quinolinyl)-4,5-dihydro-γ-oxo-1H-pyrazole-1-butanoic acid |
Tocris Bioscience |
Cat. No. 4491; CAS No: 380560-89-4 |
QNZ-46 4-[6-Methoxy-2-[(1E)-2-(3-nitrophenyl)ethenyl]-4-oxo-3(4H)quinazolinyl]benzoic acid |
Tocris Bioscience |
Cat. No. 4801; CAS No: 1237744-13-6 |
TCN-201 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl)hydrazino]carbonyl]benzyl]benzenesulfonamide |
Tocris Bioscience |
Cat. No. 4154; CAS No: 852918-02-6 |
(+)-MK 801 maleate (5S,10R)-(+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate |
Tocris Bioscience |
Cat. No. 0924; CAS No: 77086-22-7 |
MSO L-S-(3-Amino-3-carboxypropyl)-S-methylsulfoximine |
Sigma-Aldrich |
Cat. No. M5379; CAS No: 15985-39-4 |
|
Experimental Models: Organisms/Strains |
|
Grin2ctm1CreERT2_EGFP/Wtsi
|
Wellcome Trust Sanger Institute |
MGI:5632501
|
Ck1e Tau (B6.129-Csnk1etm1Asil/J) |
Jax Laboratories |
RRID: IMSR_JAX:016183 |
PER2::LUC (B6.129S6-Per2tm1Jt/J) |
Jax Laboratories |
RRID: IMSR_JAX:006852 |
|
Recombinant DNA |
|
Syn.RCaMP1h.WPRE.SV40 |
Penn Vector Core |
AV-1-PV3010 |
hSyn.GCaMP3.WPRE.SV40 |
Penn Vector Core |
AV-1-PV1627 |
hSyn.iGluSnFr.WPRE.SV40 |
Penn Vector Core |
AV-1-PV2723 |
GFAP.iGluSnFr.WPRE.SV40 |
Penn Vector Core |
AV-1-PV2726 |
hSyn.ArcLightD.WPRE.SV40 |
Penn Vector Core |
AV-1-36857P |
GfaABC1D.PI.cyto-GCaMP3.SV40 |
Penn Vector Core |
AV-5-44331 |
GfaABC1D.Lck-GCaMP6f.SV40 |
Penn Vector Core |
AV-5-PV3107 |
AAV1.CMV.PI.SynGCaMP3.SV40 |
Penn Vector Core |
AV-1-PV2368 |
CAG.Flex.tdTomato.WPRE.bGH |
Penn Vector Core |
AV-1-ALL864 |
hSyn.eGFP.WPRE.bGH |
Penn Vector Core |
AV-1-PV1696 |
GFAP.eGFP.WPRE.bGH |
Penn Vector Core |
AV-5-PV2407 |
EF1a.flex.EYFP.WPRE.hGH |
Penn Vector Core |
AV-1-27056 |
GFAP-hM3DGq:mCherry |
UNC Vector Core |
N/A |
GFAP-mCherry:Cre |
UNC Vector Core |
N/A |
Syn-mCherry:Cre |
UNC Vector Core |
N/A |
GFAP-EGFP:Cre |
UNC Vector Core |
N/A |
AAV-EF1a-flex-taCasp3-TEVp |
UNC Vector Core |
N/A |
|
Software and Algorithms |
|
BioDare |
(Moore et al., 2014) |
https://www.biodare.ed.ac.uk |
Clock Lab Analysis 6 |
ActiMetrics |
http://actimetrics.com/products/clocklab/ |
Prism 6 |
GraphPad |
http://www.graphpad.com/scientific-software/prism/ |
Igor Pro |
WaveMetrics |
https://www.wavemetrics.com/products/igorpro/igorpro.htm |
SARFIA Semi-Automated Routines for Functional Image Analysis (Igor Plugin) |
(Dorostkar et al., 2010) |
http://www.igorexchange.com/node/1723 |
Axograph X |
Axograph Scientific |
http://www.axograph.com/ |
Neuromatic (Igor plugin) |
Jason Rothman, UCL |
http://neuromatic.thinkrandom.com |
Oriana 4 |
Kovacs Computer Services |
https://www.kovcomp.co.uk/oriana/ |