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. 2017 Mar 16;2017:5629341. doi: 10.1155/2017/5629341

Figure 3.

Figure 3

S-Nitrosylation and activation level in AKT and ERK1/2. Notes. ((a) and (c)) ERK1/2 (a) and AKT (c) phosphorylation quantified by Western blotting in control and GDM placental tissue. Results are expressed as means ± SEM of phosphoprotein to total-protein ratio measured by densitometry (n = 6). Lower panels are representative images of immunoblotted proteins. ((b) and (d)) S-Nitrosylation measured in placental tissue obtained from control and GDM pregnancies. Methods used were biotin-switch technique in ERK1/2 (b) and AKT (d). Results are presented in bar charts as mean ± SEM of SNO-protein (n = 6). Lower panels are representative images of immunoblotted SNO-proteins. Units on y-axes of bar charts are arbitrary. ∗p < 0.05, GDM versus control; ∗∗p < 0.01, GDM versus control.