Fig. 1. Effects of an ethanol extract of Angelica keiskei (AK-Ex) on viable cell numbers in HepG2 cells.

Cells were plated at a density of 5 × 10⁴ cells/well in 24-well plates. One day later, (A) cells were co-treated with the indicated concentration of AK-Ex and 30 mM AAP for 48 h. (B) Cells were treated with various concentration of AK-Ex for 24 h and then treated with 30 mM AAP for 24 h. (C) Cells were treated with 30 mM AAP for 24 h and then treated with various concentration of AK-Ex for 24 h. Cell numbers were estimated by using the MTT assay. Each bar represents mean ± SEM values (n = 4). Means without a common letter differ significantly, P < 0.05.