Figure 8.

Overexpression of the CsBPCs reduces the expression of cucumber homologs of seed maturation genes. The expression levels of cucumber homologs of Em1, Em6, and 2S3 were examined in CsBPC1-cuOX#1, CsBPC3-cuOX#2, and wild-type cucumber plants. RNA was extracted from seedlings grown for 3 d and the expression levels were analyzed by qRT–PCR. Data shown are averages ± SD (n = 3). Three biological replicates were included for each experiment and 10 seedlings were included for each line. Values that differed significantly from wild type are indicated. ^** p < 0.01 by Bonferroni post-hoc test.