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. 2017 Apr 3;11:28. doi: 10.3389/fnana.2017.00028

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Copyright © 2017 Bolz, Kasper, Bufe, Zufall and Pyrski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative RT-PCR of different Na_v isoforms in main olfactory epithelium (MOE) and vomeronasal organ (VNO). Comparison of Na_v channel mRNA frequency in the (A) MOE and (B) VNO using quantitative RT-PCR. Na_v1.7 is the most abundant isoform in both subsystems and displays on average at least 5-fold higher copy numbers than other Na_v isoforms. The column diagrams show mean copy numbers ± SD from two to four independent experiments, each carried out as triplicates using total RNA of adult C57/B6 mice (Y-axis). Na_v channel isoforms (X-axis). (A) Copy numbers per ng total RNA in the MOE for Na_v1.1 (2.6 ± 0.72), Na_v1.2 (4.99 ± 0.62), Na_v1.3 (51.80 ± 7.75), Na_v1.5 (113.17 ± 27.35), Na_v1.6 (41.19 ± 10.65), Na_v1.7 (983.44 ± 221.34). (B) Copy numbers per ng total RNA in the VNO for Na_v1.1 (0.80 ± 0.42), Na_v1.2 (10.16 ± 2.10), Na_v1.3 (142.70 ± 29.05), Na_v1.5 (128.13 ± 25.56), Na_v1.6 (38.17 ± 5.39), Na_v1.7 (701.06 ± 203.50).