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. 2017 Apr 3;11:28. doi: 10.3389/fnana.2017.00028

Figure 2.

Figure 2

Analysis of Nav channel expression in MOE of adult mice. Confocal images showing immunoreactivity (red) for (A) Nav1.2, (B) Nav1.3, (C) Nav1.6, and (D) Nav1.7. We used coronal MOE cryosections (12 μm) of adult OMP-GFP mice (left, overviews; right, magnifications). Endogenous GFP (green) is located to mature, OMP+ OSNs as shown in the magnifications at the right. (A) Nav1.2 staining is restricted to microvillar cells (arrowheads) but absent from OSNs or axon bundles (dotted circles) identified by OMP-GFP labeling. (B) Robust Nav1.3 staining is present in axon bundles (arrows, left) that colocalize with OMP-GFP (dotted circles, right). (C) Nav1.6 labeling of the MOE surface (left, arrow heads) corresponds to sustentacular cells (right). OSNs and axon bundles (dotted line) lack Nav1.6 staining. (D) Nav1.7 immunoreactivity is profound in axon bundles, and occasionally found in microvillar cells (asterisk). (E,F,H,I) Blocking peptide control experiments lack immunoreactivity. (G) Nav1.3 immunoreactivity is present in cNav1.7−/− mice. (J) Nav1.7 immunoreactivity is absent in cNav1.7−/− mice. Images for each Nav channel immunostaining are representatives of n ≥ 3 mice and n = 20 sections per mouse. Scale bars overviews 100 μm, and magnifications 20 μm.