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. 2017 Apr 3;11:28. doi: 10.3389/fnana.2017.00028

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Copyright © 2017 Bolz, Kasper, Bufe, Zufall and Pyrski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Somatic expression of Na_v1.3 and Na_v1.7 occurs in mature OSNs. Colocalization of Na_v1.3, GAP43, and Na_v1.7 in the olfactory epithelium of a P7 OMP-GFP mouse (endogenous fluorescence, green). (A) Single fluorescence images for Na_v1.3 (red), OMP-GFP (green), and GAP43 (blue). (B) Magnified merge of the images in (A) shows that Na_v1.3 colocalizes with OMP-GFP (arrows) in mature OSN somata located in the apical layer (dotted line). Occasionally, triple-labeled OSNs, positive for Na_v1.3, OMP-GFP, and GAP43 were detected (asterisk). (C) Colocalization of Na_v1.3 (red) and Na_v1.7 (blue) in OMP-GFP⁺ OSNs (asterisks) intermingle with singly, OMP-GFP labeled OSNs (arrowhead). Images are representatives of (n = 2) mice with n ≥ 10 sections per mouse. Scale bars, 20 μm.