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Detection of Ac-505 in bacterial culture supernatants. (A) LC-MS chromatogram of culture supernatants from cells of the parental strain (17978) in blue and the A1S_0114 deletion derivative (Δ0114) in pink grown in static SB. The arrow indicates a compound (peak Ac-505) not present in the mutant profile. (B) Ultra high-resolution mass spectrum of HPLC purified Ac-505 by ESI FT-ICR showing the isotopic distribution of the [M+H]+ ion. (C) Proposed structure of Ac-505.
