Table 1.
Bacterial strains and plasmids used in this work.
| Strain or plasmid | Relevant characteristic(s) | Sources or references |
|---|---|---|
| STRAINS | ||
| A. baumannii | ||
| ATCC 17978 | Clinical isolate | ATCC |
| Δ0114 | ATCC 17978 A1S_0114 deletion derivative | This study |
| Δ0114.C | 17978 Δ0114 harboring pWH1266-Km-0114; Kmr | This study |
| Δ0114.E | 17978 Δ0114 harboring pWH1266-Km; Kmr, Tcr | This study |
| E. coli | ||
| TG1 | Used for DNA recombinant methods | Lucigen |
| OP50 | Used for maintenance of C. elegans; Ura−, Strr | CGC |
| PLASMIDS | ||
| pCR-Blunt II-TOPO | Cloning vector; Kmr, Zeor | Invitrogen |
| pWH1266 | A. baumannii shuttle vector; Apr, Tcr | Stiernagle, 2006 |
| pWH1266-Km | A. baumannii shuttle vector; Kmr, Tcr | This study |
| pWH1266-Km-0114 | pWH1266-Km harboring A1S_0114; Kmr | This study |
| pMo130 | Suicide vector for construction of A. baumannii isogenic derivative; Kmr, SacB, XylE | Hamad et al., 2009 |
ApR, ampicillin resistance; KmR, kanamycin resistance; TetR, tetracycline resistance; StrR, streptomycin resistance; ZeoR, zeocin resistance.