FIG 7.

Generation of MG-CreER^T2 mice. (A) Structure of the MG-CreER^T2 BAC transgenic allele. The CreER^T2 fusion cDNA was integrated by homologously replacing the 2nd to 5th exon regions of the Gata1 gene in the MG-G1 BAC DNA. Tamoxifen was administered to the mice by intraperitoneal injection on days 0, 2, 4, 5, and 6. Analysis was performed on day 14. (B) tdTomato histograms confirming Cre activity in the LSK fractions of R26T::MG-CreER^T2 and control mice. (Left) R26T::MG-CreER^T2 line 1 (red line, tamoxifen; black line, vehicle). (Middle) R26T::MG-CreER^T2 line 2 (red line, tamoxifen; black line, vehicle). (Right) R26ΔT positive control (red line) and WT negative control (black line).