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. 2017 Mar 31;37(8):e00592-16. doi: 10.1128/MCB.00592-16

FIG 8.

FIG 8

Dnmt1 deletion induces Gata1 expression and subsequently depletes HSPCs. (A) Dnmt1 and Gata1 mRNA levels in the LSK fractions from MG-CreERT2 and Dnmt1-CKO mouse bone marrows 14 days after tamoxifen treatment. (B) Bisulfite sequencing of the dbG enhancer (dbG enh) in LSK cells from MG-CreERT2 and Dnmt1-CKO adult mouse bone marrows 14 days after tamoxifen treatment. (C and D) LSK percentages (C) and LSK flow cytometry (D) for MG-CreERT2 and Dnmt1-CKO mice after tamoxifen treatment. (E and F) Late apoptotic, early apoptotic, and viable LSK cell percentages (E) and annexin V-PI staining of LSK-gated cells (F) for MG-CreERT2 and Dnmt1-CKO adult mouse bone marrows 14 days after tamoxifen treatment. Note that conditional knockout of Dnmt1 induced GATA1 expression and reduced the total LSK population and the number of viable LSK cells. Data shown are the means ± SD for three to five mice. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student's t test).