TABLE 1.
Primer and probe sequences used for genomic PCR and RT-qPCR
| Primer use and target | Sequence |
||
|---|---|---|---|
| Sense primer | Antisense primer | Probe | |
| Genotyping | |||
| MG | GGTCTCAAATGGAAGCCTGA | TGGAGTCCAGTGTCCAGTCA | |
| Chloramphenicol acetylase (BAC transgene) | CAGTCAGTTGCTCAATGTACC | ACTGGTGAAACTCACCCA | |
| GFP | CTGAAGTTCATCTGCACCACC | GAAGTTGTACTCCAGCTTGTGC | |
| ERT2 | GACAGGGAGCTGGTTCACAT | AGAGACTTCAGGGTGCTGGA | |
| Cre | ACGTTCACCGGCATCAACGT | CTGCATTACCGGTCGATGCA | |
| Dnmt1 flox | GCCAGTTGTGTGACTTGGAAA | GAGTGTGTGTTCCGTTCTCCA | |
| R26T reporter | GGCATTAAAGCAGCGTATCC | CTGTTCCTGTACGGCATGG | |
| qRT-PCR with SYBR green | |||
| Hba | CCCGGTGCCTTGTCTGCT | GGGTGAAATCGGCAGGGTGG | |
| Hbb | TGTGGGGAAAGGTGAACGC | CATGGGCCTTCACTTTGGC | |
| c-Kit | TGACAAATTCACCCTCAAAGTG | CCCCTTTCTTAAGGAGGTGAC | |
| GATA2 | ACCTGTGCAATGCCTGTGGG | TTGCACAACAGGTGCCCGCT | |
| GATA1 | CAGAACCGGCCTCTCATCC | TAGTGCATTGGGTGCCTGC | |
| Dnmt1 | CACCTTTGGTGTGCTCCAG | CGCAAACACATGCAGAGG | |
| qRT-PCR with TaqMan | |||
| rRNA | CGGCTACCACATCCAAGGAA | GCTGGAATTACCGCGGCT | TGCTGGCACCAGACTTGCCCTC |