FIG 5.

Mislocalized vesicle transport regulators in IS of Rap1b^−/− or Mst1^−/− T cells. (A and B) Localization of VPS4 and Rab11 in IS of naive OT-II T cells from wild-type and Rap1b^−/− mice. Shown are DIC and images of contact plane and 3D images (z) of TCR and VPS4 (A) or Rab11 (B). (C and D) 3D images (z) of VPS4 (C) and Rab11 (D) in Mst1^−/− naive OT-II T cells. (E) 3D images (z) of Rab8 and pMHC in naive Mst1^−/−/Mst2^−/− (Mst1/2^−/−) OT-II T cells. ***, P < 0.001; *, P < 0.05. Scale bar, 2.5 μm. Distances of the peak intensities from the contact plane were quantified and are shown on the right.