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. 2017 Mar 23;8:14813. doi: 10.1038/ncomms14813

Figure 3. Intrinsic properties of CrSAS-6 enable cartwheel ring stacking.

Figure 3

(a) Cryo-EM of CrSAS-6_NL lattice. (b) 3D representation of CrSAS-6_NL cartwheels within the lattice. Note stacked rings (blue arrow) and spokes in register (black arrows). (c) Cryo-ET section of CrSAS-6_NL lattice, highlighting eight- and nine-fold symmetrical structures, with hub (blue arrow). (d) Measurements in cryo-ET images of hub diameter (22.1±1.3 nm, n=50, only nine-fold symmetrical rings, data extracted from four tomograms) and distance from hub margin to the end of the coiled coil (36.8±1.2 nm, n=28). (e,f) The C-terminal extremity of CrSAS-6_NL forms a density visible in cryo-EM after circularization of 8-fold symmetrical cartwheels (e) or in negative stain EM (f). (g) Measurements in negative stain EM images of hub diameter (21.8±2.5 nm, n=32, one experiment) and distance from hub margin to the terminal density (37±0.9 nm, n=34, one experiment). Scale bars correspond to 50 nm.